| Autor: |
Rumiantsev DO; National Research Center for Preventive Medicine, Public Health Ministry of Russia, Moscow, Russia., Ivanova TV |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Biomedical applications [J Chromatogr B Biomed Appl] 1995 Dec 15; Vol. 674 (2), pp. 301-5. |
| DOI: |
10.1016/0378-4347(95)00310-x |
| Abstrakt: |
Sample pretreatment using solid-phase extraction (SPE) on cartridges filled with small-particle Styrosorb porous polystyrene-based sorbent has been used in the analysis of propranolol enantiomers in human serum by high-performance liquid chromatography (HPLC) with fluorescent detection. SPE on Sep-Pak C18 cartridges was used as a reference pretreatment method. The propranolol content of the samples was determined by achiral normal-phase HPLC and the enantiomeric ratio of propranolol (S/R) was then determined by chiral HPLC on a column with silica-bonded cellulose-tris(3,5-dimethylphenyl carbamate). Recoveries of propranolol from serum using SPE on Styrosorb and C18 phases were 97 +/- 5% and 96 +/- 5%, respectively. Detection and quantification limits for propranolol enantiomers were 4 and 7 ng/ml, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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