| Autor: |
Hofstadler SA; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA., Severs JC, Smith RD, Swanek FD, Ewing AG |
| Jazyk: |
angličtina |
| Zdroj: |
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 1996; Vol. 10 (8), pp. 919-22. |
| DOI: |
10.1002/(SICI)1097-0231(19960610)10:8<919::AID-RCM597>3.0.CO;2-8 |
| Abstrakt: |
Recent results with on-line capillary electrophoresis (CE) electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry suggest that CE/ESI-FTICR can provide a powerful technique for micro-sample analyses owing to the inherent sensitivity of the technique and the enhanced information content derived from high-performance mass measurements. Using micro-sampling methods and ion accumulation techniques based on quadrupolar excitation, we demonstrate that adequate sensitivity exists to characterize the hemoglobin from a single human erythrocyte (approximately 450 amol). In these studies mass spectra with average mass resolution in excess of 45 000 (FWHM) were obtained for both the alpha- and beta chain of hemoglobin following in-column lysing of a single erythrocyte. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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