| Autor: |
Franke WW; Division of Cell Biology, German Cancer Research Center, Herdelberg, Germany., Stehr S, Stumpp S, Kuhn C, Heid H, Rackwitz HR, Schnölzer M, Baumann R, Holzhausen HJ, Moll R |
| Jazyk: |
angličtina |
| Zdroj: |
Differentiation; research in biological diversity [Differentiation] 1996 Jul; Vol. 60 (4), pp. 245-50. |
| DOI: |
10.1046/j.1432-0436.1996.6040245.x |
| Abstrakt: |
We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin, and also from all other actin isoforms. We show, by immunofluorescence and immunoperoxidase microscopy of tissue sections, that cardiac/fetal alpha-actin can be localized not only in cardiomyocytes but also in skeletal muscles and their satellite cells during regeneration. These mAbs are potentially valuable in developmental biology, for the characterization of tissue and cultured myogenic cells, in pathology, and for serodiagnosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect