| Autor: |
Goodfellow IG; Department of Life Science, Nottingham University, University Park, UK., Pollitt CE, Sockett RE |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 1996 Aug 15; Vol. 142 (1), pp. 111-6. |
| DOI: |
10.1111/j.1574-6968.1996.tb08416.x |
| Abstrakt: |
A transposon mutant of Rhodobacter sphaeroides WS8 was isolated that showed reduced swarming on soft agar plates. Liquid cultures of this mutant (M18) showed a low percentage of motile swimming cells in mid-exponential phase and a low level of extracellular flagellin protein by Western blotting. M18 was complemented by a clone from a library of R. sphaeroides WS8 DNA, and restriction mapping of the site of TnphoA insertion in the mutant, coupled with DNA sequencing, showed that it had a defect in the fliI gene. To determine if a partly functional fliI gene was giving the low-motility phenotype of M18, a drug resistance omega cartridge was inserted into the gene to give a complete null mutant. This null strain also produced a low percentage of motile cells. Possible reasons for this apparent fliI-independent flagellar formation are discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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