| Autor: |
Bianchi A; Institut Alfred-Fournier, PARIS, France., Legouge R, Lefevre JC, Askienazy-Elbhar M, Joly-Guillou ML, Pavis A, Sednaoui P |
| Jazyk: |
francouzština |
| Zdroj: |
Pathologie-biologie [Pathol Biol (Paris)] 1996 May; Vol. 44 (5), pp. 347-50. |
| Abstrakt: |
In vitro activity of the 3 antimicrobial agents, against 18 recent isolates of Chlamydia trachomatis: 11 from urethral samples and 7 from endocervical samples, was tested by cell culture technique. HeLa 229 cells maintained in antibiotic-free MEM medium supplemented with 10% fetal calf serum and 2 mM glutamine were seeded into 96-well culture plates. After inoculation with 10(2) to 10(3) inclusion-forming units/ml of each strain of C. trachomatis, the culture medium was replaced by a maintenance medium containing 1 micrograms/ml of cycloheximide and serial two fold dilutions of the antibiotics. After 48 h incubation at 36 degrees C in 5% CO2 atmosphere, cells were fixed and inclusion bodies were stained using fluorescein-conjugated anti-Chlamydia monoclonal antibodies. MICs were defined as the lowest antibiotic concentrations required to inhibit the development of a single inclusion. MICs 90% (mg/l) were 0.054 for doxycyclin, 0.700 for ofloxacin and 0.150 for erythromycin. These results confirm the effective in vitro activity of the three antibiotics tested against C. trachomatis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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