| Autor: |
Windsor BL; Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48105, USA., Radulovic LL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Biomedical applications [J Chromatogr B Biomed Appl] 1995 Dec 01; Vol. 674 (1), pp. 143-8. |
| DOI: |
10.1016/0378-4347(95)00300-8 |
| Abstrakt: |
A specific and sensitive isocratic method for the measurement of a new anticonvulsant, (S)-3-(aminomethyl)-5-methylhexanoic acid, in rat plasma and milk is described. Following deproteinization, the compound and internal standard [1-(aminomethyl)cycloheptaneacetic acid] were derivatized utilizing 2,4,6-trinitrobenzene sulfonic acid and extracted with cyclohexane. Analytes were resolved on a 5 microns Spherisorb ODSII column (250 mm x 4.6 mm) using a mobile phase of 57% acetonitrile in 0.1 M ammonium acetate, pH 4.0. Absorbance was monitored at 350 nm. Limit of quantitation was 1.00 microgram/ml for a 100-microliters aliquot of plasma or milk. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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