Cardiac and renal endothelin-1 binding sites in streptozotocin-induced diabetic rats.

Autor: Vesci L; Department of Pharmacology, Sigma-Tau S.p.A., Pomezia, Italy., Mattera GG, Tobia P, Corsico N, Calvani M
Jazyk: angličtina
Zdroj: Pharmacological research [Pharmacol Res] 1995 Dec; Vol. 32 (6), pp. 363-7.
DOI: 10.1016/s1043-6618(05)80041-6
Abstrakt: Unlabelled: The aim of this work was to study cardiac and renal endothelin binding sites during the progression of diabetes. Male Crl:CD (BR) rats were made diabetic by injection of streptozotocin (STZ, 45 mg kg-1 i.v.). Only rats with a glycaemia of 500 mg per 100 ml or higher, were used. The hearts were taken at 2, 4 or 6 weeks and kidneys at 2 and 6 weeks, after diabetes induction, for binding studies. In the heart, the number of Et-1 binding sites was significantly increased 2 weeks after STZ-induction of diabetes (449 +/- 13 vs. 345 +/- 18 fmol (mg protein) -1, in controls; p < 0.05) without modification of KD value (104 +/- 5 vs 101 +/- 7 pM). Comparable results were obtained 4 and 6 weeks after STZ-induction. In the kidney both the parameters were unchanged at all the times tested.
In Conclusion: a specific increase in cardiac Et-1 binding sites, without change in affinity of the peptide, was found 2, 4 and 6 weeks after diabetes induction; while renal Et-1 binding sites were not modified.
Databáze: MEDLINE
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