Production of an extracellular polysaccharide by Agrobacterium sp DS3 NRRL B-14297 isolated from soil.

3)-, (1-->4)- and (1-->6)-linked glucose, (1-->3)- and (1-->4, 1-->6)-linked galactose and a small portion of (1-->3)-linked mannose residues. Succinic acid was not present. The molecular weight of the polysaccharide was estimated by light scattering to be 2 x 10(6) Da. The viscosity of solutions containing the polysaccharide remained constant from pH 3 to 11, and decreased by 50% when heated from 5 to 55 degrees C. Maximum yield of the polysaccharide, 20 g L-1, was reached in 48 h at 30 degrees C incubation. -->
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Substance Nomenclature: 0 (Polysaccharides, Bacterial)
IY9XDZ35W2 (Glucose)
PHA4727WTP (Mannose)
X2RN3Q8DNE (Galactose)
Entry Date(s): Date Created: 19960201 Date Completed: 19961011 Latest Revision: 20191101
Update Code: 20240829
DOI: 10.1007/BF01570073
PMID: 8730576
Autor: Hou CT; Oil Chemical Research Unit, National Center for Agricultural Utilization Research, USDA, Peoria, IL 61604, USA., Ahlgren JA, Brown W, Nicholson JJ
Jazyk: angličtina
Zdroj: Journal of industrial microbiology [J Ind Microbiol] 1996 Feb; Vol. 16 (2), pp. 129-33.
DOI: 10.1007/BF01570073
Abstrakt: A bacterium isolated from soil and identified as Agrobacterium sp produced a water-soluble extracellular polysaccharide capable of producing highly viscous solutions. Gas chromatographic analysis revealed a sugar composition of glucose, galactose and mannose in the molar ratio of 7.5:2.4:1, together with 3.7% (w/w) pyruvic acid. Methylation analyses showed the presence of (1-->3)-, (1-->4)- and (1-->6)-linked glucose, (1-->3)- and (1-->4, 1-->6)-linked galactose and a small portion of (1-->3)-linked mannose residues. Succinic acid was not present. The molecular weight of the polysaccharide was estimated by light scattering to be 2 x 10(6) Da. The viscosity of solutions containing the polysaccharide remained constant from pH 3 to 11, and decreased by 50% when heated from 5 to 55 degrees C. Maximum yield of the polysaccharide, 20 g L-1, was reached in 48 h at 30 degrees C incubation.
Databáze: MEDLINE
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