Development of instrumentation and fluorochromes for automated multiparameter analysis of cells.

Autor: Leif RC, Thomas RA, Yopp TA, Watson BD, Guarino VR, Hindman DH, Lefkove N, Vallarino LM
Jazyk: angličtina
Zdroj: Clinical chemistry [Clin Chem] 1977 Aug; Vol. 23 (8), pp. 1492-8.
Abstrakt: We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light-scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare-earth-based fluorochromes, that emit well-defined, spectrally distinct peaks.
Databáze: MEDLINE