| Autor: |
Bromke BJ; Department of Microbiology and Immunology, Philadelphia College of Osteopathic Medicine, PA, USA., Furiga MC, Hendershot RC, McGinn M |
| Jazyk: |
angličtina |
| Zdroj: |
Advances in experimental medicine and biology [Adv Exp Med Biol] 1995; Vol. 390, pp. 211-6. |
| DOI: |
10.1007/978-1-4757-9203-4_18 |
| Abstrakt: |
A protocol is presented for in vitro susceptibility testing of Trichomonas vaginalis. A 100 ml culture of the microorganisms is prepared for inoculation into antibiotic dilutions and controls by centrifugation, washing with 10 mM HEPES (pH 6.2) plus 1.5 x 10(-1) M NaCl, a second centrifugation and a resuspension in the HEPES-saline buffer. Inclusion of the gelling agent carrageenan in the culture medium permits an ease of harvesting the trichomonads and a reproducible initial cell density of 1-4 x 10(4) cells per ml. Following inoculation, tubes with antibiotic dilutions and controls are incubated anaerobically at 35 degrees C for 48 h, which corresponds to late exponential phase. Inclusion of a negative control helps determine Minimum Lethal Concentration (MLC) values. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
