A kinase-encoding gene from Colletotrichum trifolii complements a colonial growth mutant of Neurospora crassa.

Autor: Buhr TL; Department of Plant Pathology, University of Nebraska-Lincoln 68583-0722, USA., Oved S, Truesdell GM, Huang C, Yarden O, Dickman MB
Jazyk: angličtina
Zdroj: Molecular & general genetics : MGG [Mol Gen Genet] 1996 Jul 19; Vol. 251 (5), pp. 565-72.
DOI: 10.1007/BF02173646
Abstrakt: Colletotrichum trifolii is a fungal pathogen which is responsible for anthracnose disease of alfalfa. To initiate research on molecular communication in this fungus, a kinase-encoding gene (TB3) and the corresponding cDNA were cloned and sequenced. The deduced amino acid sequence of TB3 closely resembles that of a Neurospora crassa serine/threonine protein kinase, COT1, required for hyphal elongation and branching. The C-terminal catalytic domains of TB3 and COT1 are highly conserved but the N-terminal regions are divergent, particularly in the homopolymeric glutamine repeats of TB3. Northern analysis indicated that TB3 expression was highest 1 h after inducing conidial germination and 1 h before germ tubes were first observed. Expression of TB3 transcripts returned to constitutive levels by 4 h after induction of germination. TB3 complemented the cot-I mutant of Neurospora crassa, demonstrating the functional conservation of this kinase between a pathogenic and a saprophytic fungus.
Databáze: MEDLINE