| Autor: |
Iwadate Y; Department of Physics, Waseda University, Tokyo, Japan., Asai H |
| Jazyk: |
angličtina |
| Zdroj: |
Cell motility and the cytoskeleton [Cell Motil Cytoskeleton] 1996; Vol. 33 (3), pp. 175-82. |
| DOI: |
10.1002/(SICI)1097-0169(1996)33:3<175::AID-CM2>3.0.CO;2-A |
| Abstrakt: |
An improved method for the preparation of Triton X-100 extracted models of Didinium nasutum was established. Didinium models prepared by treatment with a Triton X-100 solution, containing specific proteolysis inhibitors and dimethyl sulfoxide, maintained an intact shape at 25 degrees C for a longer time than models prepared by treatment with a Triton X-100 solution not containing the proteolysis inhibitors and dimethyl sulfoxide. The improved Didinium models were reactivated so as to swim forward in the presence of Mg2+ and ATP or ADP. They did not swim backward in response to Ca2+, in contrast to well-known Paramecium models. However, the improved Didinium models showed circular swimming and spinning in response to 10(-6) M or higher concentrations of Ca2+. This observation suggests that the quick directional change as well as the spinning, which are characteristic of live Didinium, are due to an increase in the endoplasmic Ca2+ concentration around the ciliary system. The response of the Didinium ciliary system to Ca2+ may differ from the response of the Paramecium ciliary system to Ca2+. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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