| Autor: |
Kozal MJ; Department of Molecular Biology, Affymetrix, Santa Clara, California 95051, USA., Shah N, Shen N, Yang R, Fucini R, Merigan TC, Richman DD, Morris D, Hubbell E, Chee M, Gingeras TR |
| Jazyk: |
angličtina |
| Zdroj: |
Nature medicine [Nat Med] 1996 Jul; Vol. 2 (7), pp. 753-9. |
| DOI: |
10.1038/nm0796-753 |
| Abstrakt: |
Naturally occurring mutations in HIV-1-infected patients have important implications for therapy and the outcome of clinical studies. However, little is known about the prevalence of mutations that confer resistance to HIV-1 protease inhibitors in isolates derived from patients naive for such inhibitors. In the first clinical application of high-density oligonucleotide array sequencing, the sequences of 167 viral isolates from 102 patients have been determined. The DNA sequence of USA HIV-1 clade B proteases was found to be extremely variable and 47.5% of the 99 amino acid positions varied. This level of amino acid diversity is greater than that previously known for all worldwide HIV-1 clades combined (40%). Many of the amino acid changes that are known to contribute to drug resistance occurred as natural polymorphisms in isolates from patients who had never received protease inhibitors. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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