Specific depletion of the house dust mite allergen Der p 1 cereal flour prolamins.

Autor: Barber D; Alergia e Inmunología Abelló (ALK-Abelló Group), Madrid, Spain., Pernas M, Chamorro MJ, Carreira J, Arteaga C, Sánchez-Monge R, Polo F, Salcedo G
Jazyk: angličtina
Zdroj: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 1996 Apr; Vol. 97 (4), pp. 963-5.
DOI: 10.1016/s0091-6749(96)80071-6
Abstrakt: Background: Quantitation of Der 1 and Der 2 in dust samples by specific monoclonal antibodies is a method used increasingly to evaluate mite allergen exposure. The level of Der 1 has been proposed as a risk factor for sensitization.
Aim: We report a drastic decrease in the Der 1/Der 2 ratio when dust samples are collected in bakeries.
Methods: Wheat flour and purified mites were extracted simultaneously; levels of Der p 1 and Der p 2 and cysteine protease activity were determined by ELISA and inhibition experiments.
Results: High titers of Der 2, but only trace amounts of Der p 1, were detected in dust collected from bakeries. Both the level and proteolytic activity of Der p 1 appeared greatly decreased when mites and wheat flour were coextracted.
Conclusion: Group 1 protein was found to be masked by flour components, resulting in an underestimation of the mite content in bakery dust. This problem was not found for group 2 allergen.
Databáze: MEDLINE
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