| Autor: |
Petrovskaia LE, Kriukov EA, Iakimov SA, Vul'fson AN, Alibaeva RA, Shingarova LN, Guzaev AA, Abramov VM, Korobko VG |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 1995 Dec; Vol. 21 (12), pp. 912-9. |
| Abstrakt: |
Synthesis of an artificial gene encoding the signal peptide of the Yersinia pestis capsule antigen (Caf1) was accomplished. A set of plasmids coding for hybrid proteins in which a modified sequence of the Caf1 signal peptide is connected to the amino acid sequence of the mature granulocyte-macrophage colony stimulating factor (GM-CSF) were constructed. Topography of the cleavage site of signal proteases was studied. The presence of an arginine residue within the N-terminal part of the mature human GM-CSF was shown to hinder the proper processing and translocation of the precursor through periplasmic membrane. A number of E. coli strains secreting biologically active mutants of human GM-CSF were obtained. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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