| Autor: |
Neumivakin LV; Institute of Molecular Genetics, Academy of Sciences of Russia, Moscow, Russia., Solovjev VP, Sokhansanj A, Tilba VA, Moseiko NA, Shachbasian RV, Piruzian ES |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1995 Dec 26; Vol. 217 (3), pp. 796-801. |
| DOI: |
10.1006/bbrc.1995.2842 |
| Abstrakt: |
The proB proA operons (which are blocked by the feedback inhibition of proline production) of Escherichia coli wild type or with the mutation proBosm, blocking feedback inhibition effect of proline production, were cloned in a broad host range shuttle vector pVA 12-2. The hybrid plasmids pLVA(proB+A+) and pNSA(proBosm proA), were transferred into a low level osmotolerance Rhizobium fredii strain BD32. Both types of transconjugants were characterised by increased osmotolerance in a minimal medium supplied with 0.4-0.8 M NaCl but in the case of pNSA the effect was more significant. The strain BD32/pNSA had an increased level of intracellular proline concentration. Practical application of the increase in Rhizobium resistance to the stress factors is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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