| Autor: |
Bos NA; Department of Histology and Cell Biology, University of Groningen, The Netherlands., Bun JC, Popma SH, Cebra ER, Deenen GJ, van der Cammen MJ, Kroese FG, Cebra JJ |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 1996 Feb; Vol. 64 (2), pp. 616-23. |
| DOI: |
10.1128/iai.64.2.616-623.1996 |
| Abstrakt: |
We transferred peritoneal cells from BALB/c mice into C.B17 scid/scid mice. Six to eight months after injection, only cells with the B1 phenotype were retained in the spleens and peritoneal cavities of these mice. The lamina propria of the intestine contained many peritoneal, donor-derived, immunoglobulin A (IgA)-producing cells. The mesenteric lymph nodes of these mice were found to be a major site of proliferation and generation of IgA plasmablasts. We established eight IgA-producing hybridomas from the mesenteric lymph nodes of such mice, and all the hybridomas reacted with different but partially overlapping fecal bacterial populations. Cloning and sequencing of the VH genes of these hybridomas showed that two hybridomas utilized germ line-encoded VH genes while the VH genes of the six hybridomas showed somatic mutations, some of which are indicative of an antigen-driven selection process. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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