| Autor: |
Tregear JW; Department of Agricultural Sciences, University of Bristol, Long Ashton, UK., Primavesi LF, Huttly AK |
| Jazyk: |
angličtina |
| Zdroj: |
Plant molecular biology [Plant Mol Biol] 1995 Nov; Vol. 29 (4), pp. 749-58. |
| DOI: |
10.1007/BF00041165 |
| Abstrakt: |
Functional analysis of a gibberellin-regulated wheat alpha-amylase promoter, alpha-Amy2/54, has indicated that three regions were essential for expression. By studying the ability of mutant promoters, containing a randomly inserted 22 bp excision linker, to direct expression in oat aleurone protoplasts we have refined the positions and extents of these three cis elements and also demonstrated the presence of two additional elements. By converting the linker insertions to either single base point mutations or deletions using the class IIS restriction endonuclease BsmI we have shown that nucleotides -119 and -109 within the GARE -121GTAACAGAGTCTGG-108 and nucleotide -152 within the proposed element -156GATTGACTTGACC-144 are essential for high level expression from this promoter. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink