| Autor: |
Schröder U; Forschungsinstitut Borstel., Schindler C, Rüsch-Gerdes S, Kövary PM |
| Jazyk: |
němčina |
| Zdroj: |
Pneumologie (Stuttgart, Germany) [Pneumologie] 1995 Sep; Vol. 49 (9), pp. 502-4. |
| Abstrakt: |
248 respiratory specimens were examined using by Nested-PCR and by conventional methods (solid and liquid media). 54 specimens were culture-positive for M. tuberculosis, 51 of these were detected by PCR and conventional methods, whereas 3 were missed by PCR and 15 were only positive by PCR. 12 of the 15 culture-negative specimens derived from patients under treatment for tuberculosis. The 3 specimens, which are PCR negative could also be detected after modification of the method. The very high sensitivity depends on the high positive rate (21.8%) of the investigated specimens, so it is necessary to look also for other than pulmonary specimens, but it seems that his Nested-PCR is a highly specific and sensitive method for detecting of M. tuberculosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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