| Autor: |
Ciebin BW; Ontario Ministry of Health, Laboratory Services Branch, Toronto, Canada., Brodsky MH, Eddington R, Horsnell G, Choney A, Palmateer G, Ley A, Joshi R, Shears G |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 1995 Nov; Vol. 61 (11), pp. 3940-2. |
| DOI: |
10.1128/aem.61.11.3940-3942.1995 |
| Abstrakt: |
Two media used to detect fecal coliforms in water by membrane filtration, m-FC and m-TEC, were modified and supplemented with the chromogenic substrate 5-bromo-6-chloro-3-indoyl-beta-D-glucuronide (BCIG) and were compared for quantitative recovery of Escherichia coli. Student's t test of data from 181 water samples of sewage, rivers, lakes, and wells did not demonstrate any statistically significant differences (P = 0.05) in the enumeration of E. coli with these media. Target colonies were confirmed to be E. coli at rates of 98.6 and 97.3% by using FC-BCIG and TEC-BCIG media, respectively. Glucuronidase-negative isolates of E. coli were encountered at the same frequency (6.0%) on both media. This collaborative study demonstrated that either modified basal medium could be used successfully for detection of E. coli in various nontreated waters within 24 h. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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