| Autor: |
Ulsaker GA; Norwegian Medicines Control Authority, Oslo, Norway., Teien G |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical chromatography : BMC [Biomed Chromatogr] 1995 Jul-Aug; Vol. 9 (4), pp. 183-7. |
| DOI: |
10.1002/bmc.1130090407 |
| Abstrakt: |
9,10-epoxyoctadecanoic acid has been detected in human urine. Two simple purification procedures were used; the one based upon liquid-liquid extraction and the other based upon sorbent extraction technology isolating the free fatty acid fraction. Prior to trimethylsilylation and gas chromatographic-mass spectrometric analysis, both the epoxy and carboxy functions were reduced to hydroxy groups. The shift in fragmentation of a deuterated sample verified the presence of intact epoxide prior to chemical reduction. Special attention was paid to the risk of false identification of the epoxide. The content of 9,10-epoxyoctadecanoic acid in human urine was estimated to be 2.1 nM/L. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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