| Autor: |
Reeuwijk HJ; Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Netherlands., Irth H, Tjaden UR, Merkus FW, van der Greef J |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1993 Apr 21; Vol. 614 (1), pp. 95-100. |
| DOI: |
10.1016/0378-4347(93)80227-u |
| Abstrakt: |
A liquid chromatographic method using fluorescence detection for the determination of beta-cyclodextrin (beta CD) and its derivatives is presented. The chromatographic system is based on size-exclusion chromatography with the addition of the fluorophoric compound 1-naphthol to the mobile phase. Detection is based on fluorescence enhancement caused by the formation of inclusion complexes. By incorporating 10(-4) M 1-naphthol in the mobile phase, detection limits of 90, 27, 370 and 37 pmol were obtained for beta CD, hydroxypropyl-beta CD, trimethyl-beta CD and dimethyl-beta CD, respectively. The method was applied to the determination of dimethyl-beta CD in urine: the minimum detectable concentration was 0.2 microgram/ml after preconcentration of 10 ml of urine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
