| Autor: |
Vreeman HJ, Both P, Brinkhuis JA, van der Spek C |
| Jazyk: |
angličtina |
| Zdroj: |
Biochimica et biophysica acta [Biochim Biophys Acta] 1977 Mar 28; Vol. 491 (1), pp. 93-103. |
| DOI: |
10.1016/0005-2795(77)90044-7 |
| Abstrakt: |
1. A description is given of the fractionation of kappa-casein on DEAE-cellulose using a pH gradient. With this method an improved separation of the kappa-casein components with a higher negative charge is obtained. 2. It is shown that at least one of the kappa-casein fractions has a second phosphate ester group. The heterogeneity of kappa-casein therefore is not exclusively caused by a varying N-acetylneuraminic acid content. 3. Ultracentrifuge experiments and exclusion gel chromatography show that the purified kappa-casein fraction having the lowest electrophoretic mobility exhibits a monomer-polymer association equilibrium. The free energy of association per mol monomer in 0.2 M NaCl is approximately --36 kJ-mol-1. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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