| Autor: |
O'Brien T; Atherosclerosis Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905., Buithieu J, Nguyen TT, Klein L, Bren N, Wentworth M, Hallaway BJ, Kottke BA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1993 Apr 02; Vol. 613 (2), pp. 239-46. |
| DOI: |
10.1016/0378-4347(93)80138-t |
| Abstrakt: |
The aim of this paper is to describe a new methodology for the separation of human high-density lipoproteins (HDL) into apolipoprotein (apo) E-poor and apo E-rich subfractions by fast protein liquid chromatography (FPLC) using a heparin affinity column. Recoveries for apolipoproteins AI, AII, CI, CII, CIII, and E were 68.9, 74.7, 71.9, 73.5, 40.0, and 55.8%, respectively. We provide suggestive evidence that apo E-rich HDL is produced from apo E-poor HDL by the displacement of apo AI by apo E. Apo E-poor HDL was the predominant fraction. The molar ratio of apo E to apo AI in apo E-poor HDL was 0.02 and 0.01 for the subjects studied while in apo E-rich HDL it was 1.86 and 1.25. The molar ratios of the C apolipoproteins to apo AI are markedly different between the subfractions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
