| Autor: |
Torroba M; Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain., Anderson DP, Dixon OW, Casares F, Varas A, Alonso L, Gómez del Moral M, Zapata AG |
| Jazyk: |
angličtina |
| Zdroj: |
Histology and histopathology [Histol Histopathol] 1993 Apr; Vol. 8 (2), pp. 363-7. |
| Abstrakt: |
An in vitro assay was used to study the involvement of gill cells in the trapping and processing of particulate antigens. Gills were routinely processed for light microscopy after being placed in medium containing either Yersinia ruckeri O-antigen-labelled fluorescent beads, unlabelled fluorescent beads, Y, ruckeri O-antigen or formalin-killed Y. ruckeri, for 0, 30 s, 1, 5 and 30 min. Y. ruckeri formalin-killed cells, Y. ruckeri O-antigen and fluorescent beads labelled with Y. ruckeri O-antigen were taken in by gill epithelial cells as soon as 30 s after administration. In contrast, unlabelled fluorescent beads adhered to the epithelial cell membranes, but did not occur inside the gill cells. These results are discussed principally in relationship with the specificity of antigen trapping. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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