| Autor: |
Mikhaĭlov AT, Gorgoliuk NA, Mukhoian MM |
| Jazyk: |
ruština |
| Zdroj: |
Ontogenez [Ontogenez] 1993 Mar-Apr; Vol. 24 (2), pp. 24-32. |
| Abstrakt: |
The data are presented about isolation from the brain of 7.5-day chick embryos of a factor capable of neuralizing effect on the early gastrula ectoderm of the grass frog Rana temporaria L. Earlier this factor was defined as embryonic brain-derived neuralizing factor (EBDNF) (Mikhailov, Gorgoliuk, 1989). The isolation procedure includes (1) extraction with deionized water at pH 9.0; (2) ion exchange chromatography on a column with DEAE-adsorbent at pII 8.0; (3) affinity chromatography on Heparin-Ultragel column. EBDNF-containing fraction is eluted from the Heparin-Ultragel column with 250 mM NaCl as a separate peak. Four bands are observed on SDS-electrophoregrams of this fraction, two more prominent ones having the molecular weight of 43 and 63 kDa. The yield of EBDNF-containing fraction is about 0.01-0.02% of the wet weight of the initial brain tissue. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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