| Autor: |
Wilson D; Research Department, Ciba-Geigy Corp., Summit, New Jersey 07901., Paul PK, Roberts ED, Blancuzzi V, Gronlund-Jacob J, Vosbeck K, DiPasquale G, O'Byrne EM |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) [Proc Soc Exp Biol Med] 1993 May; Vol. 203 (1), pp. 30-7. |
| DOI: |
10.3181/00379727-203-43569 |
| Abstrakt: |
Cartilage pathology in rabbit knees was monitored by noninvasive magnetic resonance imaging (MRI) and evaluated using morphometric histologic measurements. Infusion of rabbit knees with the cytokine interleukin 1 induces cartilage degradation and inflammation. A miniosmotic pump was implanted subcutaneously to deliver interleukin 1 through a polyethylene catheter inserted into the rabbit knee. Rabbit knees were imaged using MRI and prepared for histologic examination at 5 and 12 days after chronic infusion of interleukin 1. MRI obtained 0.7-mm sections for three-dimensional reconstruction of cartilage image. Cartilage deterioration near the site of infusion was visible on MRI. MRI measurements indicated a reduction in cartilage thickness. Histology revealed a loss of staining of cartilage matrix proteoglycan, synovial hypertrophy, and perichondral bone resorption. Morphometric analysis of cartilage histology indicated a reduction in both cellularity (chondrocytes/m mu 2 area) and cell to matrix area ratio. These observations suggest that a loss of proteoglycan, an early event in cartilage degeneration, can be detected by MRI. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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