| Autor: |
Tanner GJ; CSIRO Division of Plant Industry, Canberra, ACT, Australia., Kristiansen KN |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 1993 Mar; Vol. 209 (2), pp. 274-7. |
| DOI: |
10.1006/abio.1993.1119 |
| Abstrakt: |
A novel method is presented for the synthesis and purification of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin. Soluble enzyme extracts from developing barley grains and leaves of the forage legume Onobrychis viciifolia (sainfoin) catalyzed the NAD(P)H-dependent reduction of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin to (+)-[4-3H]catechin. NADPH was the preferred substrate. With extracts of barley the rate of reaction with 1 mM NADH was 20% of the rate found with NADPH. With extracts from both tissues there was a broad pH optimum around pH 6.6. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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