| Autor: |
Stiff DD; Department of Pharmacy and Therapeutics, School of Pharmacy, University of Pittsburgh Medical Center, PA 15261., Frye RF, Branch RA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1993 Mar 05; Vol. 613 (1), pp. 127-31. |
| DOI: |
10.1016/0378-4347(93)80205-i |
| Abstrakt: |
A rapid and sensitive high-performance liquid chromatographic assay was developed for the quantitation of chlorzoxazone and its major metabolite 6-hydroxychlorozoxazone in plasma. These compounds, as well as the internal standard 5-fluorobenzoxazolone, were extracted from plasma (0.5 ml) using C18 solid-phase extraction columns. The extracts were analyzed on a 10-microns Waters C18 muBond-apak column with a mobile phase of acetonitrile-tetrahydrofuran-0.1 M ammonium acetate (22.5:5.5:72). The assay utilized ultraviolet detection (283 nm) which provided sensitivity and specificity sufficient to simultaneously quantify > or = 100 ng/ml chlorozoxazone and 6-hydroxychlorzoxazone in plasma. The mean correlation coefficient of the multi-level standard curves for each compound was 0.996 or greater over a concentration range of 100-3000 ng/ml. The inter-day and intra-day coefficients of variation were < 6%. This method has been used by our laboratory to provide the unattended overnight analysis of chlorozoxazone and 6-hydroxychlorzoxazone in plasma samples obtained from human study subjects. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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