Correlation of specific virus-astrocyte interactions and cytopathic effects induced by ts1, a neurovirulent mutant of Moloney murine leukemia virus.

Ile, in precursor envelope protein gPr80env. This Val-25-->Ile substitution apparently renders gPr80env inefficient for transport from the endoplasmic reticulum to the Golgi apparatus. These findings suggest that the cytopathic effect of ts1 in neural cells might be due to the accumulation of gPr80env in the endoplasmic reticulum. Since endothelial and glial cells are targets of ts1 infection in the central nervous system, we established primary endothelial and astrocyte cultures to investigate the mechanism of cell killing caused by ts1. A continuous cell line, TB, was used as a control. Our results showed that both ts1 and MoMuLV-TB replicated and induced a cytopathic effect in astrocyte cultures, albeit to different degrees; ts1 appeared to be more lethal than MoMuLV-TB. On the other hand, ts1 and MoMuLV-TB infections of endothelial or TB cells were not cytopathic. The cytopathic effect in infected astrocytes correlated with the inefficiency of gPr80env transport and the intracellular accumulation of gPr80env as well as aberrant virus particles. -->
References: J Exp Med. 1988 Apr 1;167(4):1428-41. (PMID: 3258626)
AIDS. 1987 Dec;1(4):229-34. (PMID: 3126770)
Science. 1988 Nov 11;242(4880):919-22. (PMID: 2460922)
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9580-4. (PMID: 2849111)
Virology. 1989 Jun;170(2):450-9. (PMID: 2728346)
J Virol. 1989 Sep;63(9):3748-54. (PMID: 2788223)
Brain Res. 1989 Jul 10;491(2):394-7. (PMID: 2765895)
J Virol. 1990 Feb;64(2):467-75. (PMID: 2296075)
J Acquir Immune Defic Syndr. 1990;3(7):658-68. (PMID: 2161920)
Curr Top Microbiol Immunol. 1990;160:29-60. (PMID: 2162285)
J Virol. 1990 Oct;64(10):4661-71. (PMID: 2204721)
J Virol. 1990 Nov;64(11):5241-9. (PMID: 2214016)
Virology. 1990 Dec;179(2):749-58. (PMID: 1700541)
Virology. 1991 Jan;180(1):365-71. (PMID: 1984656)
J Virol. 1991 Apr;65(4):2047-55. (PMID: 1900540)
J Exp Med. 1991 Apr 1;173(4):981-91. (PMID: 2007861)
Virology. 1991 Jul;183(1):12-9. (PMID: 1711254)
Virus Res. 1991 Mar;19(1):83-92. (PMID: 1867010)
Virology. 1991 Oct;184(2):687-94. (PMID: 1887590)
Virology. 1992 Jan;186(1):303-8. (PMID: 1309276)
J Virol. 1992 Apr;66(4):2296-301. (PMID: 1548763)
Virology. 1992 May;188(1):25-32. (PMID: 1314453)
AIDS. 1992 Mar;6(3):273-85. (PMID: 1373627)
Nature. 1992 Aug 27;358(6389):761-4. (PMID: 1380673)
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20. (PMID: 4861258)
Virology. 1973 Feb;51(2):424-31. (PMID: 4735036)
Virology. 1981 Mar;109(2):366-78. (PMID: 6259818)
Virology. 1983 Mar;125(2):513-8. (PMID: 6836918)
J Virol. 1985 Apr;54(1):178-85. (PMID: 2983112)
J Virol. 1985 Sep;55(3):760-7. (PMID: 4020966)
Annu Rev Biochem. 1985;54:631-64. (PMID: 3896128)
J Virol. 1986 Apr;58(1):67-74. (PMID: 3005660)
Am J Pathol. 1986 Sep;124(3):457-68. (PMID: 3766704)
J Virol. 1987 Apr;61(4):1244-7. (PMID: 3644020)
FEBS Lett. 1987 Mar 9;213(1):138-43. (PMID: 3549356)
Proc Natl Acad Sci U S A. 1987 May;84(10):3526-30. (PMID: 3472222)
J Virol. 1987 Dec;61(12):3774-82. (PMID: 2446007)
J Virol. 1988 Aug;62(8):2578-86. (PMID: 3260631)
Grant Information: 16672 United States PHS HHS; AI 28283 United States AI NIAID NIH HHS; CA 45124 United States CA NCI NIH HHS
Substance Nomenclature: 0 (Protein Precursors)
0 (Viral Envelope Proteins)
Entry Date(s): Date Created: 19930301 Date Completed: 19930323 Latest Revision: 20200724
Update Code: 20240829
PubMed Central ID: PMC237478
DOI: 10.1128/JVI.67.3.1137-1147.1993
PMID: 8437206
Autor: Shikova E; University of Texas M. D. Anderson Cancer Center, Smithville 78957., Lin YC, Saha K, Brooks BR, Wong PK
Jazyk: angličtina
Zdroj: Journal of virology [J Virol] 1993 Mar; Vol. 67 (3), pp. 1137-47.
DOI: 10.1128/JVI.67.3.1137-1147.1993
Abstrakt: ts1 is a highly neuropathogenic and lymphocytopathic mutant of Moloney murine leukemia virus TB (MoMuLV-TB). We previously reported that the primary neuropathogenic determinant of ts1 maps to a single amino acid substitution, Val-25-->Ile, in precursor envelope protein gPr80env. This Val-25-->Ile substitution apparently renders gPr80env inefficient for transport from the endoplasmic reticulum to the Golgi apparatus. These findings suggest that the cytopathic effect of ts1 in neural cells might be due to the accumulation of gPr80env in the endoplasmic reticulum. Since endothelial and glial cells are targets of ts1 infection in the central nervous system, we established primary endothelial and astrocyte cultures to investigate the mechanism of cell killing caused by ts1. A continuous cell line, TB, was used as a control. Our results showed that both ts1 and MoMuLV-TB replicated and induced a cytopathic effect in astrocyte cultures, albeit to different degrees; ts1 appeared to be more lethal than MoMuLV-TB. On the other hand, ts1 and MoMuLV-TB infections of endothelial or TB cells were not cytopathic. The cytopathic effect in infected astrocytes correlated with the inefficiency of gPr80env transport and the intracellular accumulation of gPr80env as well as aberrant virus particles.
Databáze: MEDLINE
Externí odkaz: