Autor: |
Long GW; Center for Immunization Research, Johns Hopkins University, Baltimore, Maryland 21205., Oprandy JJ, Narayanan RB, Fortier AH, Porter KR, Nacy CA |
Jazyk: |
angličtina |
Zdroj: |
Journal of clinical microbiology [J Clin Microbiol] 1993 Jan; Vol. 31 (1), pp. 152-4. |
DOI: |
10.1128/jcm.31.1.152-154.1993 |
Abstrakt: |
We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture. |
Databáze: |
MEDLINE |
Externí odkaz: |
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