| Autor: |
Moore ED; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester 01605., Etter EF, Philipson KD, Carrington WA, Fogarty KE, Lifshitz LM, Fay FS |
| Jazyk: |
angličtina |
| Zdroj: |
Nature [Nature] 1993 Oct 14; Vol. 365 (6447), pp. 657-60. |
| DOI: |
10.1038/365657a0 |
| Abstrakt: |
The Na+/Ca2+ exchanger, driven by a transmembrane Na+ gradient, plays a key role in regulating Ca2+ concentration in many cells. Although the exchanger influences Ca2+ concentration, its activity in smooth muscle appears to be closely coupled to Ca2+ availability from intracellular stores. This linkage might result if the exchanger were positioned close to Ca2+ storage sites within the sarcoplasmic reticulum. To test this hypothesis we have developed methods to assess the relative three-dimensional distribution of proteins involved in Na+/K+ pumping, Na+/Ca2+ exchange, Ca2+ storage within the sarcoplasmic reticulum, and attachment of contractile filaments to the membrane in smooth muscle. Here we report that the Na+/Ca2+ exchanger is largely co-distributed with the Na+/K+ pump on unique regions of the plasma membrane in register with, and close to, calsequestrin-containing regions of the sarcoplasmic reticulum in sites distinct from the sites where contractile filaments attach to the membrane. This molecular organization suggests that the plasma membrane is divided into at least two functional domains, and appear to provide a mechanism for the strong linkage seen in smooth muscle between Na+/K+ pumping and Na+/Ca2+ exchange, and between Na+/Ca2+ exchange and Ca2+ release from the sarcoplasmic reticulum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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