| Autor: |
Griffin HG; Genetics and Microbiology Department, AFRC Institute of Food Research, Colney, Norwich, UK., I'Anson KJ, Gasson MJ |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 1993 Aug 15; Vol. 112 (1), pp. 49-53. |
| DOI: |
10.1111/j.1574-6968.1993.tb06422.x |
| Abstrakt: |
A method for the direct screening of bacterial lambda libraries by polymerase chain reaction technology has been developed. This technique permits the identification and isolation of specific DNA sequences without the need for any filter hybridisation or radioactive probing. This strategy has been used to isolate a gene encoding lactate dehydrogenase from a Lactococcus lactis lambda library. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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