Identifying protein kinases in crude extracts that phosphorylate cyclic GMP-binding cyclic-GMP specific phosphodiesterase.

Autor: Corbin JD; Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232., Woodall CC, Colbran JL, McAllister LM, Sekhar KR, Francis SH
Jazyk: angličtina
Zdroj: Agents and actions. Supplements [Agents Actions Suppl] 1993; Vol. 43, pp. 27-33.
DOI: 10.1007/978-3-0348-7324-6_3
Abstrakt: The main protein kinase that phosphorylates cyclic GMP-binding cyclic GMP-specific phosphodiesterase (cG-BPDE) in crude extracts of bovine lung is cyclic GMP-dependent protein kinase. This can be shown by the use of either exogenous or endogenous cG-BPDE as substrate for endogenous cyclic GMP-dependent protein kinase. The characteristics of this phosphorylation suggest a physiological significance.
Databáze: MEDLINE