| Autor: |
Estacion M; Molecular Oncology Program, Cancer Research Center of Hawaii, Honolulu., Mordan LJ |
| Jazyk: |
angličtina |
| Zdroj: |
Cell calcium [Cell Calcium] 1993 Feb; Vol. 14 (2), pp. 161-71. |
| DOI: |
10.1016/0143-4160(93)90085-k |
| Abstrakt: |
Platelet derived growth factor (PDGF) mobilizes multiple calcium pools in the C3H 10T1/2 mouse fibroblast, including a sustained influx of extracellular calcium. We have used the whole cell patch-clamp technique to directly test for a role of plasma membrane calcium channels in this influx. Whole-cell patch-clamp recordings revealed a voltage-gated calcium channel with gating properties consistent with a 'T-type' designation. This phenotype of the C3H 10T1/2 fibroblasts was dependent upon the cell density in culture. The fraction of cells expressing calcium channels was low (< 10%) in subconfluent culture but rose to approximately 50% as the cells established a confluent monolayer. The magnitude of the PDGF-stimulated sustained calcium influx component measured using Fura-2 increased in parallel with the expression of calcium current. We interpret these results to support the hypothesis that T-type voltage-gated calcium channels contribute to the PDGF-stimulated intracellular calcium signals in these cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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