Rabbit esophageal cells possess K+ channels: effect of hyposmotic stress on channel activity.

Autor:	Goldstein JL; Department of Medicine, University of Illinois, Chicago., Fogelson BG, Snow JC, Schmidt LN, Mozwecz H, Layden TJ
Jazyk:	angličtina
Zdroj:	Gastroenterology [Gastroenterology] 1993 Feb; Vol. 104 (2), pp. 417-26.
DOI:	10.1016/0016-5085(93)90409-6
Abstrakt:	Background: In many cell types, basolateral K+ channels are important in maintaining transepithelial Na+ absorption and regulatory volume decrease (RVD) after hyposmolar stress. However, in the esophagus the effect of K+ transport in maintaining baseline short-circuit current (SCC) (Na+ absorption) and RVD is unknown. Methods: Ussing chambers were used to evaluate changes in SCC of rabbit esophageal mucosa in response to serosal Ba2+ (4 mmol/L), quinine (1 mmol/L), and increasing serosal [K+]. To determine whether K+ channel(s) are activated in RVD, changes in SCC in response to serosal hyposmolarity (156 mOsm) were assessed in the presence or absence of serosal quinine. Results: Serosal Ba2+, quinine, or increased serosal [K+] caused a decline in baseline SCC. Serosal hyposmolarity caused an increase in SCC that was not blocked by mucosal application of amiloride (10(-4) mmol/L). In contrast, serosal quinine completely blocked the hyposmolar-induced increase in SCC. Conclusions: These studies suggest that rabbit esophageal cells possess Ba(2+)- and quinine-sensitive basolateral K+ channel(s) that are active under baseline conditions. Potassium conductance(s) also appear to be activated by external serosal hyposmolarity and may be involved in the process of RVD.
Databáze:	MEDLINE
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