| Autor: |
Ranalder UB; Department of New Technologies, Hoffmann-La Roche AG, Basel, Switzerland., Lausecker BB, Huselton C |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1993 Jul 23; Vol. 617 (1), pp. 129-35. |
| DOI: |
10.1016/0378-4347(93)80431-3 |
| Abstrakt: |
The separation and quantitation of the pentafluorobenzyl derivatives of all-trans- and 13-cis-retinoic acids and their 4-oxo metabolites in human plasma on micro high-performance liquid chromatographic columns (0.32 mm I.D.) is described. The column outlet was directly coupled to the source of a quadrupole mass spectrometer via a simple SFC-frit interface. Negative ion chemical ionization conditions were obtained by coaxial introduction of ammonia as a reagent gas. A signal-to-noise ratio well above 3 was obtained for 1 pg of each analyte injected. The limit of quantitation calculated from spiked biological plasma extracts was 0.3 ng/ml. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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