Progesterone receptor messenger ribonucleic acid and protein are overexpressed in human uterine leiomyomas.
| Autor: | Brandon DD; Department of Medicine, Oregon Health Sciences University, Portland 97201-3098., Bethea CL, Strawn EY, Novy MJ, Burry KA, Harrington MS, Erickson TE, Warner C, Keenan EJ, Clinton GM |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | American journal of obstetrics and gynecology [Am J Obstet Gynecol] 1993 Jul; Vol. 169 (1), pp. 78-85. |
| DOI: | 10.1016/0002-9378(93)90135-6 |
| Abstrakt: | Objective: Our purpose was to identify molecular mechanisms underlying abnormal growth of uterine leiomyomas. Study Design: Biopsy samples of tumor and adjacent "normal" myometrium from nine patients were analyzed for progesterone receptor gene expression and for proliferation-associated antigen Ki-67. Results: Northern analysis indicated that progesterone receptor messenger ribonucleic acid levels were increased twofold to 15-fold in leiomyoma compared with adjacent myometrial biopsy tissue from all patients (n = 9), whereas beta-actin messenger ribonucleic acid was at similar levels in these samples. Quantitative immunoassay, immunohistochemistry studies, and Western blot analyses revealed increased amounts of progesterone receptor protein in the tumor tissue. Both the progesterone receptor A and B forms were expressed in the leiomyoma and adjacent myometrium. Corresponding to increased progesterone receptor gene expression, the proliferation-associated antigen Ki-67 was also significantly elevated in the leiomyoma tissue. Conclusion: These data provide the first evidence that progesterone receptor messenger ribonucleic acid is overexpressed in uterine leiomyomas, suggesting that amplified progesterone-mediated signaling is instrumental in the abnormal growth of these tumors. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|