| Autor: |
Kuypers AW; Department of Internal Medicine, University Hospital Nijmegen, Netherlands., Willems PM, van der Schans MJ, Linssen PC, Wessels HM, de Bruijn CH, Everaerts FM, Mensink EJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1993 Nov 24; Vol. 621 (2), pp. 149-56. |
| DOI: |
10.1016/0378-4347(93)80090-q |
| Abstrakt: |
The use of capillary electrophoresis (CE) in a polymer network for single-strand conformation polymorphism (SSCP) is investigated. SSCP is a method to detect DNA point mutations, essential in the diagnosis of several diseases. The PCR (polymerase chain reaction) amplified p53 gene, a tumour suppressor gene known to be frequently mutated in malignant cells, was subjected to CE analysis. Two single-strand DNA fragments of 372 bp in length differing in only one nucleotide could be separated. We conclude that SSCP using CE in a polymer network is a powerful method for the detection of point mutations in DNA sequences. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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