| Autor: |
Albensi BC; Neuroscience Program, University of Utah, Salt Lake City 84132., Ryujin KT, McIntosh JM, Naisbitt SR, Olivera BM, Fillous F |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroreport [Neuroreport] 1993 Sep 30; Vol. 4 (12), pp. 1331-4. |
| DOI: |
10.1097/00001756-199309150-00011 |
| Abstrakt: |
The peptide toxin omega-conotoxin GVIA (omega-CgTx) has been shown to be a high affinity ligand for N-type calcium channels in the brain. We have employed [125I]omega-CgTx to localize N-type channels in human hippocampus and cerebellum using autoradiography. Ten micron thick slide-mounted tissue sections of human cerebellum and hippocampus were labeled with [125I]omega-CgTx under various conditions. Specific binding to human cerebellum was virtually irreversible and saturable. It was displaceable by the N-channel antagonist, omega-conotoxin MVIIA, but not by L- or P-channel ligands. Binding sites were heterogeneously distributed with denser binding in the molecular layer than the granule cell layer of cerebellum and with specific laminar patterns evident in the hippocampus. [125I]omega-CgTx should be a useful tool for the study of N-type calcium channels in human brain tissue. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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