| Autor: |
Christensen T; Novo Nordisk A/S, Novo Allé, Bagsvaerd, Denmark., Petersen J, Theisen CF, Bjerregaard K, Kristensen SM, Led JJ |
| Jazyk: |
angličtina |
| Zdroj: |
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) [Acta Chem Scand (Cph)] 1993 Oct; Vol. 47 (10), pp. 990-3. |
| DOI: |
10.3891/acta.chem.scand.47-0990 |
| Abstrakt: |
Biosynthetic human growth hormone (hGH) specifically 15N labelled in the leucine residues has been obtained by recombinant DNA technology, using 15N-labelled leucine and an E. coli strain that requires leucine. It is shown that, despite the possibility of minor transaminase activity, the labelling on the whole is specific, and that the two-dimensional 1H-15N correlation NMR spectra of hGH can be greatly simplified by this methodology. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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