| Autor: |
Richa; Department of Veterinary Microbiology, Haryana Agricultural University, Hisar, India., Grover YP, Charan S |
| Jazyk: |
angličtina |
| Zdroj: |
Revue scientifique et technique (International Office of Epizootics) [Rev Sci Tech] 1993 Sep; Vol. 12 (3), pp. 923-30. |
| DOI: |
10.20506/rst.12.3.728 |
| Abstrakt: |
The authors describe a rapid and simple dot immunobinding assay (DIA) for detection and identification of equine herpesvirus-1 antigen in field samples from cases of abortion, stillbirth, perinatal foal mortality and paralysis. The assay employs a nitrocellulose membrane to which antigen is adsorbed as a dot. Antigen is identified as a coloured dot using a procedure based on the principle of enzyme-linked immunosorbent assay (ELISA). In all, 61 samples were tested by DIA and the test was compared with conventional agar gel immunodiffusion (AGID). With DIA, 44 (72%) samples gave positive results, while only 22 of 61 (36%) samples tested positive by AGID. DIA was observed to be rapid, more sensitive and more specific than AGID, in addition to the obvious advantage of being reagent-conservative, inexpensive and simple to perform. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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