| Autor: |
Thomsen C; Novo Nordisk A/S, Pharmaceuticals Division, Måløv, Denmark., Frandsen A, Suzdak PD, Andersen CF, Schousboe A |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroreport [Neuroreport] 1993 Sep 10; Vol. 4 (11), pp. 1255-8. |
| DOI: |
10.1097/00001756-199309000-00011 |
| Abstrakt: |
The cytotoxic potential of (1S,3R)-1-amino-cyclopentane-1,3-dicarboxylic acid (t-ACPD) in cultured murine cerebral cortical neurones was examined. Exposure to t-ACPD (1 mM, 16 h) did not induce cytotoxicity per se or affect N-methyl-D-aspartate-induced toxicity. Phosphoinositide hydrolysis was weakly stimulated by t-ACPD (1 mM), while no alteration of intracellular calcium levels was observed after exposure to 1 mM t-ACPD. However, forskolin-stimulated cAMP formation was inhibited by t-ACPD (IC50 = 8 +/- 2 microM) with a maximal inhibition of 36 +/- 4% of control levels. These data suggest that the lack of neurotoxic effects of t-ACPD may be related to the negligible efficacy of t-ACPD to activate mGluRs coupled to the IP3/Ca(2+)-cascade and/or putative counteracting effects of t-ACPD mediated by mGluRs negatively coupled to the cAMP-cascade. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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