| Autor: |
Hakovirta H; Department of Anatomy, University of Turku, Finland., Vierula M, Wolpe SD, Parvinen M |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1994 Feb; Vol. 99 (1), pp. 119-24. |
| DOI: |
10.1016/0303-7207(94)90154-6 |
| Abstrakt: |
To find out whether macrophage inflammatory protein-1 alpha (MIP-1 alpha) has a role in the regulation of germ cell development, we studied its effects on spermatogenic stage-specific DNA synthesis in vitro. MIP-1 alpha increased the DNA synthesis of primitive type A2-4 spermatogonia and of premeiotic cells, whereas the DNA synthesis of more differentiated intermediate and type B spermatogonia was inhibited when cultured in the presence of MIP-1 alpha. An antibody against MIP-1 alpha cross-reacted with a protein of 15 kDa from every spermatogenic stage of rat seminiferous epithelium. Immunohistochemical studies with the same antibody revealed a complex pattern of MIP-1 alpha localization both in primitive and advanced spermatogenic cells. These observations suggest that MIP-1 alpha is a local regulator of mitotic and meiotic DNA synthesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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