| Autor: |
Britsyna NS, Prokop'ev AA, Kulikov SV, Samartsev MA |
| Jazyk: |
ruština |
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 1994 Mar-Apr; Vol. 30 (2), pp. 316-20. |
| Abstrakt: |
An enzyme immunoassay (ELISA) for the detection of human proinsulin has been developed based on the interaction of the absorbed proinsulin with the antiserum against the synthetic proinsulin C-peptide. In the presence of high concentrations of insulin, the sensitivity of the assay slightly decreases due to the competitive adsorption of insulin onto the polystyrene carrier with the binding constant 800-1000 times less than that of proinsulin. A method is proposed for the interpretation of ELISA data based on analysis of a weighed dry insulin sample, which enables the detection of a 0.02-0.1% admixture of proinsulin in the chromatographically purified recombinant human insulin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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