| Autor: |
Ramasamy R; Division of Life Sciences, Institute of Fundamental Studies, Kandy, Sri Lanka., Wickremaratne C |
| Jazyk: |
angličtina |
| Zdroj: |
The Indian journal of medical research [Indian J Med Res] 1994 Jan; Vol. 99, pp. 21-6. |
| Abstrakt: |
The specificity of murine antibodies raised against structurally related peptides derived from a malaria parasite membrane protein was studied. The peptides were conjugated to bovine serum albumin (BSA) with 6-maleimido caproic acyl N-hydroxysuccinimide ester before immunization. Conjugation to BSA through a C-terminal or an internal cysteine residue elicited antibodies with noticeably different specificities. An N-terminal tripeptide sequence arginine-asparagine-asparagine had a dominant influence on the immunogenicity of the peptides. Such factors need to be taken into consideration while designing peptide-based immunogens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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