Photoaffinity cross-linking of F1ATPase from spinach chloroplasts by 3'-arylazido-beta-alanyl-8-azido ATP.

Autor: Schäfer HJ; Institut für Biochemie, Johannes Gutenberg-Universität, Mainz, Germany., Rathgeber G, Schuhen A, Berzborn RJ
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 1994 Mar 07; Vol. 340 (3), pp. 265-8.
DOI: 10.1016/0014-5793(94)80151-7
Abstrakt: UV irradiation of the ATPase (CF1) from spinach chloroplasts in the presence of 3'-arylazido-beta-alanyl-8-azido ATP (8,3'-DiN3ATP) results in a nucleotide-dependent inactivation of the enzyme and in a nucleotide-dependent formation of alpha-beta cross-links. The results demonstrate an interfacial localization of the nucleotide binding sites on CF1.
Databáze: MEDLINE