Production of fertile transgenic maize by electroporation of suspension culture cells.

Autor: Laursen CM; Discovery Research, DEKALB Plant Genetics, Mystic, CT 06355-1958., Krzyzek RA, Flick CE, Anderson PC, Spencer TM
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 1994 Jan; Vol. 24 (1), pp. 51-61.
DOI: 10.1007/BF00040573
Abstrakt: Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.
Databáze: MEDLINE