Identification of low-molecular-mass coproantigens of Cryptosporidium parvum.

Autor: el-Shewy K; Division of Pediatric Infectious Diseases, University of Alberta, Edmonton, Canada., Kilani RT, Hegazi MM, Makhlouf LM, Wenman WM
Jazyk: angličtina
Zdroj: The Journal of infectious diseases [J Infect Dis] 1994 Feb; Vol. 169 (2), pp. 460-3.
DOI: 10.1093/infdis/169.2.460
Abstrakt: Cryptosporidium parvum coproantigens (CCAg) of 18 and 20 kDa were identified in the stool eluates of calves and humans infected with Cryptosporidium species. Monospecific antibodies raised against the 20-kDa antigen recognized both 18- and 20-kDa CCAg in all positive but no negative control samples. These antibodies reacted with C. parvum sporozoites in an immunofluorescence assay. Human immune sera recognized the 20-kDa antigen in infected calf stool eluates. Both 18- and 20-kDa CCAg remained intact in commonly used preservatives and at various temperatures. These CCAg may be useful in designing sensitive, reliable methods for diagnosing cryptosporidiosis.
Databáze: MEDLINE