| Autor: |
Allioli N; Centre de Génétique Moléculaire et Cellulaire, CNRS UMR106, INRA LA810, Université Claude Bernard Lyon-I, Villeurbanne, France., Thomas JL, Chebloune Y, Nigon VM, Verdier G, Legras C |
| Jazyk: |
angličtina |
| Zdroj: |
Developmental biology [Dev Biol] 1994 Sep; Vol. 165 (1), pp. 30-7. |
| DOI: |
10.1006/dbio.1994.1231 |
| Abstrakt: |
Three methods of isolating primordial germ cells (PGCs) from gonads of 5-day-old chick embryos were compared. PGCs were then cultured in vitro in DMEM/F12 medium containing 10% fetal calf serum. BrdU incorporation showed that at least 10% of the PGC population were dividing, under our culture conditions, during the 2nd day of in vitro culture. During this culture period, PGCs were exposed to avian leukosis sarcoma virus-based retroviral vector pseudotyped with subgroup A envelope, carrying the LacZ reporter gene. X-Gal staining showed that PGCs were permissive to infection, with more than 50% of PGCs expressing the beta-Gal protein. These data represent the first demonstration that PGCs, isolated from gonads of 5-day-old chick embryos, are able to divide in vitro and that it is possible to introduce and express exogenous DNA in chick PGCs maintained in vitro. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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